MENU
In this lab, we study plant-microbe interactions with a particular interest in cytology and epigenetics of fungal pathogens. We elucidate various aspects of plant-microbe interactions such as adhesion of a fungal pathogen to the surface of a plant leaf, cell differentiation of fungal pathogens during infection, and plant defense mechanisms against pathogens. The research and educational activities are aimed at elucidation of the underlying molecular mechanisms and application of research findings for human benefit. Especially, we also try to develop an environmental friendly measure of disease protection such as biological control.
We focused on the causal agent of blast disease of gramineous plants, Pyricularia oryzae. Its spore is very small ca. 30×10 μm, and elaborates an appressorium after germination on the host plant to penetrate into a host cell.
Epigenetic genome regulation in phytopathogenic fungi
During the course of infection, phytopathogenic fungi including the blast fungus, Pyricularia oryzae, form various morphologically and functionally different cells such as spores, germination tubes, and appressoria. Such cell differentiation during infection is assumed to be controlled by epigenetic regulation such as histone modifications and DNA methylation. We are focusing on this point and performing research in details. In the figure below, we investigated changes in the levels of H3K4me2 and H3K4me3, which are typical epigenetic marks on histone, during the course of infection, referring to those of RNA expression. In addition, we are also working on epigenetic regulation of transposable elements that can be a cause of a new fungal pathotype by generating genetic variations in the host fungal genome.
